IS 14988 : Part 1 : 2020/ISO 11290-1 : 2017 Microbiology of the Food Chain-Horizontal Method for Detection and Enumeration of Listeria monocytogenes and of Listeria spp. - Part 1 Detection Method

Revised Standard from Last Update.

NATIONAL FOREWORD

This Indian Standard (Part 1) (First Revision) which is identical with ISO 11290-1 : 2017 ‘Microbiology of the food chain — Horizontal method for detection and enumeration of Listeria monocytogenes and of Listeria spp. — Part 1: Detection method’ issued by the International Organization for Standardization (ISO) was adopted by the Bureau of Indian Standards on recommendation of the Food Hygiene, Safety Management and Other Systems Sectional Committee and approval of the Food and Agriculture Division Council.

This standard was originally published in 2001 and was identical with ISO 11290-1 : 1996. The First revision of this standard has been undertaken to align it with the latest version of ISO 11290-1 : 2017.

The major changes in this revision are as follows:

a) The detection of Listeria monocytogenes has been modified as listed below:

1) Primary enrichment in half-Fraser broth: incubation for 25 h ± 1 h.

2) Secondary enrichment in Fraser broth: incubation for 24 h ± 2 h.

3) Half-Fraser and Fraser broths may be refrigerated before transfer or isolation on selective agar for a maximum of 72 h.

4) Storage of isolation plates: incubated plates can be refrigerated for a maximum of two days before reading.

5) Microscopic aspect for confirmation is optional if the isolation agar allows distinction between pathogenic and non-pathogenic Listeria spp.

6) CAMP test and catalase test are optional.

7) Inclusion of new performance characteristics.

b) Moreover, detection of Listeria spp. has been included in the scope and the title changed accordingly.

This Indian Standard is published in two parts. The other part in this series is:

Part 2 Enumeration method

The text of ISO Standard has been approved as suitable for publication as an Indian Standard without deviations. Certain terminologies and conventions are, however, not identical to those used in Indian Standards. Attention is particularly drawn to the following:

a) Wherever the words ‘International Standard’ appear referring to this standard, they should be read as ‘Indian Standard’.

b) Comma (,) has been used as a decimal marker, while in Indian Standards, the current practice is to use a point (.) as the decimal marker.