IS 19308 : 2025 Determination of Vitamin B12 in Food Using Liquid Chromatography and Tandem Mass Spectrometry (LC-MS/MS) - Method of Test

1 SCOPE

This standard specifies the method for the quantitative determination of vitamin B12 forms, specifically cyanocobalamin and methylcobalamin, in food matrices such as fortified Atta (flour), almonds and similar matrices, using the UHPLCMS/ MS technique. Its application to other food matrices requires appropriate laboratory verification or validation, as applicable.

2 REFERENCE

This standard given below contain provisions which, through reference in this text, constitute provisions of this standard. At the time of publication, the editions indicated was valid. All standards are subject to revision and parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent edition of this standard: