IS 5401 : Part 2 : 2012/ISO 4831 : 2006 Microbiology - Food and animal feeding Stuffs- Horizontal method for the Detection and Enumeration of Coliforms Part 2 Most probable Number Technique

Reaffirmed 2022

NATIONAL FOREWORD

This Indian Standard (Part 2) (Second Revision) which is identical with ISO 4831 : 2006 ‘Microbiology of food and animal feeding stuffs - Horizontal method for the detection and enumeration of coliforms - Most probable number technique’ issued by the International Organization for Standardization (ISO) was adopted by the Bureau of Indian Standards on the recommendation of the Food Hygiene, Safety Management and Other Systems Sectional Committee and approval of the Food and Agriculture Division Council.

This standard was originally published in 1969. The first revision of this standard was undertaken in 2002 to align with the earlier versions of ISO Standards on the subject, namely, ISO 4831 : 1991 ‘Microbiology - General guidance for the enumeration of coliforms - Most probable number technique’ and ISO 4832 : 1991 ‘Microbiology - General guidance for the enumeration of coliforms - Colony count technique’. Accordingly, first revision of Part 1 of IS 5401 covered general guidance for enumeration of coliforms by colony-count technique, which was identical with ISO 4832 : 1991 and first revision of Part 2 of IS 5401 covered general guidance for enumeration of coliforms by most probable number technique, which was identical with ISO 4831 : 1991. The second revision of this standard has been undertaken to align it with the latest version of the International Standard, namely, ISO 4831 : 2006.

The text of ISO Standard has been approved as suitable for publication as an Indian Standard without deviations. Certain conventions are, however, not identical to those used in Indian Standards. Attention is particularly drawn to the following:

a) Wherever the words ‘International Standard’ appear referring to this standard, they should be read as ‘Indian Standard’.

b) Comma (,) has been used as a decimal marker while in Indian Standards, the current practice is to use a point (.) as the decimal marker.