IS 5835 : 2025 Determination of Vitamin D in Fortified Vegetable Oils by Ultra-High Performance Liquid Chromatography and Tandem Mass Spectrometry (UHPLC-MS/MS) - Method of Test

1 SCOPE

This standard specifies a method for the quantitative determination of vitamin D forms in vegetables oils by ultra-high performance liquid chromatographytandem mass spectrometry (UHPLC-MS/MS). This standard is not intended to be used for products which are not fortified by vitamin D. This method is applicable for edible oils which contain vitamin D2 or vitamin D3 in the range of 10 μg/kg to 200 μg/kg.

2 REFERENCE

The standard given below contain provisions which, through reference in this text, constitute provisions of this standard. At the time of publication, the editions indicated was valid. All standards are subject to revision and parties to agreements based on this standard are encouraged to investigate the possibility of applying the most recent edition of this standard: